The effect of the WKYMVm peptide on promoting mBMSC secretion of exosomes to induce M2 macrophage polarization through the FPR2 pathway

Abstract Background When multicystic vesicles (precursors of exosomes) are formed in cells, there two results. One is decomposition by lysosomes, and the other generation exosomes that transported out through transmembrane. On hand, M2 macrophages promote formation local vascularization provide necessary support for repair bone defects. To a new idea treatment defects, purpose our study was to investigate effect WKYMVm (Trp-Lys-Tyr-Met-Val-D-Met-NH2) peptide on secretion from murine marrow-derived MSCs (mBMSCs) polarization macrophages. Methods The used activate formyl receptor 2 (FPR2) pathway mBMSCs. First, we Cell Counting Kit-8 (CCK-8) detect cytotoxic Second, western blotting (WB) quantitative real-time polymerase chain reaction (qRT-PCR) expression interferon stimulated gene 15 (ISG15) transcription factor EB (TFEB) Then, detected lysosomal activity using lysozyme assay kit. Third, an exosome extraction kit content secreted Fourth, immunofluorescence count number polarized Finally, inhibitor block miRNA-146 mBMSCs counted Results We first found had no toxic at concentration 1 ?mol/L. when FPR2 activated mBMSCs, ISG15 TFEB decreased, leading increased exosomes. also decreased demonstrated Moreover, all these effects can be blocked WRWWWW (WRW4, H-Trp-Arg-Trp-Trp-Trp-Trp-OH) peptide, pathway. confirmed promoting Conclusion Our findings potential value eventually macrophage polarization. believe could research basis clinical